Dead sperm stain


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LIVE/DEAD Cell Viability Assays

We studied consecutive semen samples delivered to our clinical laboratory mainly men in couples consulting the clinic due to infertility problems, primarily for diagnosis, secondarily for treatment, but also men referred for endocrine disturbances affecting the testicular function. It is always the case that some sperm from an ejaculate are morphologically abnormal, but when that fraction becomes excessive, fertility may decrease. The nigrosin-stain stain produces a dark background on which the sperm stand out as lightly colored objects. Secondary defects are less serious and thought to arise during passage thought the epididymis or by mishandling after ejaculation. This may be due to immaturity of the ejaculated sperm or to a functional defect in the tail or midpiece. This small portion is combined with a drop of red stain on a slide. This staining procedure produces very nice preparations, but is more difficult and time consuming that nigrosin-eosin.
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LIVE/DEAD Cell Viability Assays | Thermo Fisher Scientific - US

Abnormalities can be classified as affecting the head, midpiece or tail. This should be evaluated with a hemocytometer. The technique was used on washed human sperm by Mortimer Mortimer et al. This can be a very important test for a couple and their physician who are trying to determine how best to approach their infertility. It is also useful to subclassify the abnormal population into the types of abnormality observed.
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JorVet J-326S Live-Dead Semen Stain

Dead sperm stain



Description: This staining procedure produces very nice preparations, but is more difficult and time consuming that nigrosin-eosin. Sixty sperm appear white or not stained. Primary defects are the more severe and are thought to originate while the sperm was still within the semeniferous epithelium of the testis. Comparison of four different techniques for vitality assessment with dye exclusion. Have microscope slides and nigrosin-eosin stain prewarmed to body temperature.

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